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木霉工程菌株酯酶同工酶和可溶性蛋白的电泳分析

The Electrophoresis Analysis of Esterase Isozymes and Soluble Proteins of Transformed Trichoderma Strains

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【Author】 HUANG Yu-qian1,CHEN Jie1,2,HAN Mei1,LIANG Chun-hao3(1.Shenyang Agricultural University,Shenyang 110161,China;2.Shanghai Jiaotong University,Shanghai 201101,China; 3.Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

【机构】沈阳农业大学；辽宁省农业科学院沈阳110161；沈阳110161；上海交通大学；上海201101；

【摘要】用聚丙烯酰胺凝胶电泳技术对木霉菌的野生菌株T21及其4株REMI转化子T31、T34、T47、T55的可溶性蛋白和酯酶同工酶进行了比较研究。结果表明各转化子之间、转化子与野生菌株之间的谱带均有差异。野生菌株T21及转化子T31、T34、T47、T55的蛋白谱带为22,21,19,17,24条;酯酶谱带为6,8,4,5,3条。更多还原

【Abstract】 The soluble proteins and esterase isozyme of Trichoderma wild strain T21 and REMI transformed strains T31,T34,T47,T55 were analysed with polyacrylamide gel electrophoresis.The results showed that there were differences among transformed strains,between wide strain and transformed strains.The soluble proteins band amounts of wild strain T21 and REMI transformed strains T31,T34,T47,T55 were 22,21,19,17and24.The esterase isozyme band amounts was 6,8,14,15and3.更多还原

【关键词】木霉；酯酶同工酶；可溶性蛋白；
【Key words】 Trichoderma； esterase isozyme； soluble proteins；

【文献出处】沈阳农业大学学报 ,Journal of Shenyang Agricultural University , 编辑部邮箱 ,2007年03期

【分类号】S476.1
【下载频次】208

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